By Diponkar Banerjee (auth.), Diponkar Banerjee, Sohrab P. Shah (eds.)

ISBN-10: 1627032800

ISBN-13: 9781627032803

ISBN-10: 1627032819

ISBN-13: 9781627032810

The earliest descriptions of human chromosomes initiated the genomics revolution that's now upon us. Array Comparative Genomic Hybridization: Protocols and Applicationsexplores the scope of what's now attainable so far as documenting abnormalities linked to a number of human cancers. whereas the know-how for interrogating the human genome maintains to adapt, refinement of comparative genomic hybridization (CGH) utilizing array CGH and comparable applied sciences have supplied huge, immense perception into human cancers at a cheap scale. Written within the winning Methods in Molecular Biology™ sequence layout, chapters contain introductions to their respective themes, lists of the required fabrics and reagents, step by step, without problems reproducible protocols, and notes on troubleshooting and warding off identified pitfalls.

Authoritative and simply available, Array Comparative Genomic Hybridization: Protocols and Applicationsprovides researchers with well-honed methodologies to profit those strategies for his or her personal use in study or scientific diagnostic laboratories.

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Extra resources for Array Comparative Genomic Hybridization: Protocols and Applications

Example text

Figure 1 shows two examples of sorting performed with different specimens. 3. After sorting keep the nuclear suspension on ice. Do not freeze and proceed with the isolation of DNA as soon as possible. 4. Isolation of DNA from Nuclei and Determination of DNA Quality It would be useful at this point to read carefully the manual published by Agilent, which describes and discusses in depth the experimental protocols and various options (18). The following sections describe experimental choices used in our laboratory.

Cytogenet Genome Res 105:18–24 17. Pugh TJ, Delaney AD, Farnoud N et al (2008) Impact of whole genome amplification on analysis of copy number variants. Nucleic Acids Res 36:e80 18. Agilent Technology, Inc. pdf 19. Agilent Technology, Inc. pdf 20. Agilent Technology, Inc. pdf 21. Agilent Technology, Inc. pagetype=Cust om&subpagetype=Custom&pageid=2151, resource on the web. 22. Agilent Technology, Inc. pdf 23. Hicks J, Krasnitz A, Lakshmi B et al (2006) Novel patterns of genome rearrangement and their association with survival in breast cancer.

It is possible to obtain enough nuclei from needle core biopsies with tumor content less than 70% as long as the core biopsy is at least 1mm ´ 5mm in size. 5. It is important to make sure that blood is collected from the patients before or after the biopsy procedure. Blood lymphocytes could be the source of matched normal DNA. 6. Flow sorting of nuclei is an excellent way to obtain pure tumor DNA from the dominant subpopulation in the specimen. However, this step can be skipped and DNA can be extracted directly from the frozen biopsy sample at the risk of having nonhomogenous DNA.

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Array Comparative Genomic Hybridization: Protocols and Applications by Diponkar Banerjee (auth.), Diponkar Banerjee, Sohrab P. Shah (eds.)

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