By Marina S. Turishcheva (auth.), Yu. S. Nasyrov, Z. à esták (eds.)
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Additional info for Genetic Aspects of Photosynthesis
The ct-r-RNA sequences in the pea ct-DNA accounted for about 4% of the DNA in a number of different experiments. Taking the molecular weight of pea ct-DNA as 90 x 106 , this level of hybridization indicates the presence of two r-RNA cistrons in the ct-DNA. sr-------------------------------. 8 ct · DNA filter. II" 3 4 5 1'9 RNA PER 1'9 DNA Fig. 14. Hybridization of pea ct· DNA ttl pea ct-r-RNA. 0 ml of 4 X sse for 16 h. 0 m1 of 20 Ilg m1- 1 RNase in 2 X sse at 25 °e for 2 h and washed with 60 ml of 2 X sse on each side.
119: 62p, 1970. LOEB, J. , Biochim. biophys. Acta 157 : 424, 1968. LOWRY, O. , ROSEBROUGH, N. , FARR, A. L. & RANDALL, R. , 1. bioi. Chem. 193: 265,1951. MAKINO, F. , Nature 231 : 446, 1971. , 1. mol. Bioi. 3 : 208, 1961. MARMUR, J. ,Nature 183: 1427,1959. MOHBERG, J. & RUSCH, H. , Arch. Biochem Biophys. 134 : 577, 1969. NETRAWALI, M. , Exp. Cell Res. 63 : 422, 1970. PINEVICH, V. V. & ZHDAN-PUSHKINA, S. , Vestn. iening. gos. , Ser. bioi. 9 (2) : 109,1972. RAAF, J. & BONNER. , Arch. Biochem Biophys.
Information Content in the Ct-DNAs The information content in the ct-DNA for the ct-ribosomal (r-)RNA has been analyzed by DNA-RNA hybridization using labeled RNA. A. Number ofr-RNA Cistrons Purified 70S ribosomes of the chloroplast were obtained from in vivo labeled plants. The r-RNA was isolated from these ribosomes by the method described in the legend of Fig. 13. The fractionation of r-RNA in linear sucrose gradients (15-30%) yielded 23S and l6S RNAs in the ratio of 2: 1, respectively. The sizes of r-RNA were confirmed by co-electrophoresing them with labeled r-RNA from Escherichia coli, and the purity of the fractionated 23S and l6S r-RNA was analyzed by electrophoresis in acrylamide gels.
Genetic Aspects of Photosynthesis by Marina S. Turishcheva (auth.), Yu. S. Nasyrov, Z. à esták (eds.)