By Dongyou Liu

ISBN-10: 1420076434

ISBN-13: 9781420076431

Whereas the majority of our nutrients offers are nutritious and secure, foodborne pathogen-related ailment nonetheless impacts hundreds of thousands of individuals every year. huge outbreaks of foodborne ailments — akin to the new salmonella outbreak associated with quite a few peanut butter items — stay said with alarming frequency. All-Encompassing advisor to Detection innovations the most potent how one can keep an eye on and forestall human foodborne infections is to enforce a pathogen surveillance procedure. Addressing the biology, epidemiology, and pathogenesis of greater than 60 microorganisms, Molecular Detection of Foodborne Pathogens demonstrates the best way to enforce a surveillance procedure that makes use of cutting-edge molecular ideas to speedy notice and determine bacterial, fungal, viral, and parasitic pathogens involved. incorporated in each one bankruptcy: Concise assessment of the chosen pathogen in recognize to its biology, epidemiology, and pathogenesis precis of molecular detection tools on hand Description of clinical/food pattern assortment and training approaches collection of powerful, powerful, step by step detection protocols dialogue of the present demanding situations and ongoing learn must additional expand software and function of molecular diagnostic equipment With confirmed, ready-to-use protocols (including advertisement kits), this globally pertinent source demonstrates how quickly and powerful detection tools may end up in extra lives kept and a continuously secure, caliber, and reliable meals offer.

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Extra resources for Molecular Detection of Foodborne Pathogens

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Numerous molecular techniques have emerged that offer the advantage of speed along with specific and sensitive detection. Molecular methods have also proven advantageous in cases where it is difficult to culture the target of interest, as can be the case with viruses. These methods require a solid understanding of the physiology of the target organism, its close relatives, and those with which it may coexist on a food surface. 5 Foodborne pathogens have been associated with a wide variety of foods including poultry, beef, shellfish, fruits, vegetables, and drinking water.

These techniques may use several sets of primers or more than one enzyme to carry out amplification of the target product without thermal cycling. 67 NASBA is a sensitive detection method for the detection of RNA or DNA. The reaction typically consists of three enzymes including T7 RNA polymerase, deoxyribonucleoside triphosphates, two specific primers, and buffering reagents and takes place at approximately 40°C. 70 Jean et al. 4 ng RNA/ml as compared to a reverse transcriptase PCR assay used that yielded a detection limit of 4 ng RNA/ml.

The carA gene which encodes carbamoyl phosphate synthase in Pseudomonas sp. 170 In order to differentiate between different strains of E. 171,172 For the detection of Salmonella in poultry houses the iroB gene, which is absent in the closely related E. 173,174 The cpn60 gene (also known as groEL or hsp60), which encodes a heat shock protein in bacteria, contains within it a fragment that has been useful for determining phylogenetic relationships among bacteria. 175 The rpsU- dnaG- rpoD region is another locus has been used to differentiate between different bacteria.

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Molecular Detection of Foodborne Pathogens by Dongyou Liu

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