By Borek Janik

ISBN-10: 1461346126

ISBN-13: 9781461346128

ISBN-10: 1461346142

ISBN-13: 9781461346142

Physicochemical experiences on po1ynuc1eotides and their elements are a comparatively new box which, nearly day-by-day, draws an ever­ expanding variety of scientists. so far, in spite of the fact that, just a restricted attempt has been made to bring together the gigantic quantity of physicochemical facts on hand right into a necessary layout. I initially undertook this compilation of information to complement my very own examine efforts. however it quickly grew to become obtrusive that my labors could be of profit to different employees during this advanced quarter, and this quantity is the outcome. the knowledge pointed out during this manuscript covers the literature as much as the tip of 1970. the current compilation can't potentially checklist all recorded dissociation constants, extinction coefficients, and Tm values of po1ynuc1eotides, oligonucleotides, and their com­ plexes. As defined within the "Explanation of Tables," a few discre­ tion needed to be exercised within the choice of oligonucleotides and po1ynuc1eotides for inclusion. each attempt has been made, notwithstanding, to supply greater than only a consultant pass part of the on hand literature.

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Additional info for Physicochemical Characteristics of Oligonucleotides and Polynucleotides

Sample text

H. 1. OLIGONUCLEOTIDES 01igomers G-U (Con 't) pH d(T-A) Ex 10- 3 1/mo1·cm h Note Ref. 08 ~ KC10 4 ,K phosp. h. h. 19 (0. 0 252, 320 115 N 260* 11. 4 e Amax (2)= 260 32 162 54 2. SPECTRAL DATA mfl. 2 d (T-C) 273 21. 8 h Note Ref. h. h. 1. OLIGONUCLEOTIDES 01igomers U-C (Con't) Medium pH A mfJ. E x 10- 3 1/mo1·cm h % Note Ref. 1 !! 7!! Ol!! 1 !! 6 260* dU-G 2 258 U-Gp 1 O. I!! 05 !! phosp. 05 !! phosp. 1 !! 7 !! Ol!! h. 28)n 55 A-A-C 1 259 11. 3 (11. 5 28 56 01igomers 2. 0 Ref. 1 phosp. h. 2 ! 05 ~ phosp.

Tris 261. h. 7!! Ol!! 1 !! 2 B. 7!! Ol!! 2 B. 7!! Ol!! Tris 261. h. h. l!! l!! l!! 1 !! 6 C-Gp A mp. 1 aC-A C-G Medium 25° 33 150 m 4 2. 1 M HC1 A ml1 260* E x 10- 3 1/mo1'cm Note Ref. i phosp. i phosp. 9 H2O d(pC-G) 2;5 max aC-dN 2;5 max dC-T 2 273 21. 2 2 273 21. 2 1 hl(225), 1. 00th0. 1. OLIGONUCLEOTIDES Oligomers C-U (Con't) 51 Medium pH A m/.! 6 C-A 1 Note Ref. h. 0 25 0 ! h. h. c 167 260* 4. 5 4. h. h. h. 3" c 33 c c 167, 169 167, 169 32 Amax (2)= 257 162 52 01igomers G-C 2. 1 !! HC1 A mJ.!

0 (11. h. 1 !! 1!! 1 !! 05 !! phosp. 05 !! phosp. 1 !! 20 4 4 112 m 4 2. 14 Ref. h. 5 260* 1 258 11. 5 28 7 260* 11. 3(11. 1 ~ phosp. 5 260 11. h. 1. 1. 0 A. 03 ~ phosp. 05 ~ phosp. 3(260) NaC1 Ref. lMKOH 261 31. 1 M KOH 260* 31. 4 7 O. 05 ~ phosp. 05 ~ phosp. l1 m 11. h. 89)n 284 60 01igomers C-C-Gp 2. SPECTRAL DATA Medium pH A mJ.! 1 l:! 1 l:! 05 l:! phosp. 05 l:! phosp. 1 l:! 5(260) 24 4 112 4 4 ll2 250 (255, 284 280),1. 20 (1. h. 02 l:! 01 l:! h. 5 11. 2 (11. 8 260* Ref. h 28 28 237 1. 08 HC104 260* 11.

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Physicochemical Characteristics of Oligonucleotides and Polynucleotides by Borek Janik


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